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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Dev Biol. 2010 Sep 15;347(2):337–347. doi: 10.1016/j.ydbio.2010.08.029

Figure 4. PKA inactivation conversely stimulates UB outgrowth from the WD.

Figure 4

WDs after two days of culture with cell-permeable inhibitors specific for PKA: (A) control (B) Rp-8-CPT-cAMPS 10µM (C) KT-5720 1µM (D) PKAinhibitor(14–22) 10µM (E–F) Quantitative morphometric analysis confirms that the Wolffian ducts cultured in the presence of PKAinhibitor(14–22) generated a significantly greater number of ureteric buds per standardized length of WD as well as significantly greater surface area per bud, when compared to control. n=10 WD cultures (* = p < 0.01). Scale bar = 250µm.