Table 2.
List of antibodies for immunocytochemistry
| MS |
LPS |
||||
|---|---|---|---|---|---|
| Primary antibody |
Target | Source | Primary antibody |
Target | Source |
| Myelin damage | |||||
| α- MAG D7E10 | Myelin associated glycoprotein |
Doberson et al. (1985) | α- MAG D7E10 | Myelin associated glycoprotein |
Doberson et al. (1985) |
| α- MOG | 8-18C5 Myelin oligodendrocyte glycoprotein |
Linington et al. (1988) | α- MOG 8-18C5 |
Myelin oligodendrocyte glycoprotein |
Linington et al. (1988) |
| α-PLP | Proteolipid protein |
Serotec, UK MCA 839G | α-PLP | Proteolipid protein | Serotec, UK MCA 839G |
| α- CNPase | Cyclic nucleotide phosphodiesterase |
Affiniti CA 1130 | α- CNPase | Cyclic nucleotide phosphodiesterase |
Affiniti CA 1130 |
| Adaptive immunity | |||||
| α-CD3 | T-cells | DAKO Glostrup DK A 0452 |
W3/13 | Leukosialin; T-cells & Granulocytes |
Harlan Sera-Lab Loughborough, UK |
| α-CD4 | MHC II restricted T-cells |
Neomarkers Freemont CA Ab8 |
|||
| α-CD8 | MHC I restricted T-Cells |
DAKO C8/144 | |||
| Axonal damage | |||||
| α- NF | neurofilament | Chemicon AB 1981 | α- NF | neurofilament | Chemicon AB 1981 |
| α- APP | Amyloid Precurser Protein |
Chemicon; Temecula CA MAB 348 |
α- APP | Amyloid Precurser Protein |
Chemicon Temecula CA MAB 348 |
| Microglia-/macrophage activation | |||||
| α- Hc10 | MHC-I; α-chain | Stam et al. (1990) | |||
| α- β2M | MHC-I | DAKO A 072 | α-β2M | MHC-I | Santa Cruz sc-8361 |
| α- HLA- DR | MHC-II | DAKO CR 3/43 | α-OX6 | MHC-II | Serotec MRC Ox6 |
| α- iNOS | Inducible nitric oxide synthetase |
Chemicon Temecula CA AB 5384 |
α-NOS II | Inducible nitric oxide synthetase |
Chemicon Temecula CA AB 1631 |
| α-CD68 | Activated macro- phages/microglia |
DAKO MO 814 | α-ED1 | Activated macrophages/ microglia |
Serotec MCA 341 |
| α-CD163 | Scavenger receptor | Novo Castra Newcastle, UK NCL-CD163 |
α-EMAP II | Endothelial-monocyte- activating peptide II |
Abcam Cambridge UK Ab 15693 |
| α-CD14 | LPS receptor | Neomarkers Clone 14C02 |
|||
| α-AIF-1 (iba-1) | Allograft inflamma- tory factor-1 |
Wako 01-1974 | α-AIF-1 (iba-1) | Allograft inflammatory factor-1 |
Wako 01-1974 |
| α-MPO | Myelo peroxidase | DAKO A 0398 | α-MPO | Myelo peroxidase | DAKO A 0398 |
| Tissue preconditioning | |||||
| α-HIF-1 α | Hypoxia inducible factor1 α |
BD biosciences Pharmingen |
|||
| α-Hsp70 | Heat shock protein70 |
Stressgen Victoria, Canada SPA-810 |
α-Hsp70 | Heat shock protein70 | Stressgen, Victoria, Canada SPA-810 |
| BBB damage | |||||
| α- fibrin | Fibrinogen | US Biological F4200-06 | α- fibrin | fibrin | US Biological F4200-06 |
| Complement activation | |||||
| PC #42 | C9neo | S. Piddlesden Univ Cardiff, UK |
|||
| Rabbit α Rat | C9 | Rabbit α Rat | C9 | S. Piddlesden | |
| MC B7 | C9neo | ||||