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. 2010 Nov 15;21(22):3890–3901. doi: 10.1091/mbc.E10-06-0520

Figure 8.

Figure 8.

Mutations in the conserved IE of p107 enhance expression. (A) Similarities between Rbf1 IE and homologous region of p107, which is most similar to Rbf1. Asterisks mark basic residues mutated in each protein to stabilize expression. (B) Genes for Flag-tagged wild-type p107 or IE mutants were transfected into S2 cells and expression quantitated by Western blot. The 60-aa region deleted from p107 in Δ964-1024 is similar to the Rbf1 IE. Endogenous tubulin levels are shown as controls.