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. 2010 Nov 15;21(22):3934–3941. doi: 10.1091/mbc.E10-06-0513

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 3. — Jen1p facilitates selenite uptake. Yeast strains BY4741 (●), QZ1 (○), QZ1pDR196 (▾), and QZ1pJEN1 (▵) were grown in minimal SD medium supplied with either 2% galactose or glucose to exponential phase (OD₆₀₀ ∼1.0). The cells were harvested and suspended in transport buffer. Sodium selenite was added to the yeast suspensions at a final concentration of 1 mM, and at the indicated times portions (0.1 ml) were passed though the membrane filters. The filters were digested, and total selenium was quantified by ICP-MS. Three repeats of each were used to derive the means and SEs.