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Jen1p catalyzes high-affinity uptake of both selenite and lactate. Uptake of selenite (A) or lactate (B) by strain QZ1pJEN1 was determined after 30 min, as described under Materials and Methods. The values from the deletion strain QZ1 were subtracted at each time point to correct for nonspecific binding. The Km and Vmax values were determined using a hypobolic fit of the data with SigmaPlot.
