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(A) Selenite uptake as a function of pH. Selenite transport in QZ1pJEN1was assayed at the indicated pHs at a final concentration of 1 mM. (B) FCCP and nigericin inhibit selenite uptake. Cells of QZ1pJEN1 (black bar) were incubated with FCCP (100 μM) or nigericin (300 μM) for 5 min before addition of selenite at a final concentration of 1 mM, compared with QZ1 (white bar). Selenium accumulation was measured after 30-min incubation.
