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. 2010 Nov 15;21(22):3926–3933. doi: 10.1091/mbc.E10-02-0112

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2. — Redifferentiation is not promoted by (additional) HGF treatment in activated Raf:ER cells. Raf:ER cysts were treated with 4HT alone for 48 h, and HGF was added or not for additional 24 h. (A) In the absence of 4HT, HGF-induced cysts formed tubules with mature lumens. (B) Cysts treated with 4HT alone for 72 h exhibited cell dispersion. GFP fluorescence reflects Raf:ER induction. (C) Treatment with HGF plus 4HT failed to complete tubule development. Quantification of cysts with tubule(s) (bottom) (mean ± SEM, n = 3). Bar, 10 μm.