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Overexpression of tyrosine phosphorylation defective STAT1 partially rescues cord formation of activated Raf:ER cysts. (A) Raf:ER cysts (left) and three clones overexpressing Y701F-STAT1 were treated with 4HT for 72 h. Bar, 10 μm. (B) Total lysate from Raf:ER cells and three Y701F-STAT1 transfectants were immunoblotted for FLAG tag on Y701F-STAT1. (C) Quantification of cords in the presence of 4HT (mean ± SEM, n = 3). *p < 0.05 and **p = 0.06).
