Skip to main content
. 2010 Nov 15;21(22):3985–3997. doi: 10.1091/mbc.E10-05-0456

Figure 5.

Figure 5.

Coimmunoprecipitation of c-NKCC2 and MAL/VIP17 in CTR and low Cl conditions. LLC-PK1 cells expressing c-NKCC2 and MAL/VIP17 were incubated in CTR or low Cl conditions, lysed in 2% CHAPS, and subjected to immunoprecipitatation with anti-FLAG antibody. Western blotting analysis was made with R5 (A) or T4 (B) antibodies. The phosphorylation of the cotransporter in low Cl did not change the apparent affinity of c-NKCC2 for MAL/VIP17.