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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Cell Physiol. 2011 Jan;226(1):132–140. doi: 10.1002/jcp.22314

Fig. 5.

Fig. 5

Bmal1 regulates expression of vWF. A: Western blot analysis of the levels of vWF in plasma samples from WT and Bmal1−/− mice collected every 4 h during the 24-hr period. ZT—zeitgeber time, ZT0 corresponds to the lights-on time, ZT12—lights-off time. The graph shows densitometry analysis of the data. B: Expression of vWF mRNA in aortic tissue of WT and Bmal1−/−. C: Daily average levels of vWF mRNA in the liver of WT and Bmal1−/− mice. D: vWF promoter activity in HEK293 cells transfected with different combinations of CLOCK, Bmal1, and CRY1 expression plasmid. E: ChIP assay of vWF promoter and PAI-1 promoters in isolated murine lung endothelial cells demonstrating direct interaction of Bmal1 with the E-box sequence.