Figure 2.

Reorganization of Secretory Pathway Organelles after Enteroviral Infection

HeLa cells (A) coexpressing Arf1-RFP/ɛCOP-YFP; expressing Arf1-GFP (B, C, E–I); or coexpressing Arf1-RFP/GalT-YFP (see Movie S2) (D) were infected with CVB3 for 4 hr. Cells in (B), (C), (E)–(I) were fixed and coimmunostained with anti-GFP and (B) anti-clathrin heavy chain; (C) anti-γ-adaptin; (E) anti-GM130; (F) anti-TGN46; (G) anti-GGA1; (H) anti-Rab1b; (I) anti-ERGIC53 antibodies. Arrows in (A), (B), (C), and (D) indicate Arf1-labeled membranes that do not label with ɛCOP-YFP, clathrin, γ-adaptin, and GalT-YFP, respectively. See also Figures S3A–S3H and Figure S4.

All fluorescence images were confocal images of optical slice thickness ∼1 μm. Scale bar, 10 μm.