Assay for BMMCs	Cells per well/100 μl	Incubation time	Antigen	Anticipated release
β-hexosaminidase	3–5×104	30 min	10 ng/ml	20–50%
LTC4	100	30 min	10 ng/ml	700–1200 pg/ml
PGD2	2×103	30 min	10 ng/ml	90–200 pg/ml
Cytokine	1×105	6 h	10 ng/ml	IL-6: 300–600 pg/ml TNFα: 100–200 pg/ml
Chemokine	1×105	6 h	10 ng/ml	MCP-1: 200–300 pg/ml MIP1-α: 400–800 pg/ml