| Assay for HuMCs | Cells per well/100 μl | Incubation time | Antigen | Anticipated release |
|---|---|---|---|---|
| β-hexosaminidase | 0.5–1×104 | 30 min | 10 ng/ml | 20–60% |
| LTC4 | 200 | 30 min | 10 ng/ml | 100–130 pg/ml |
| PGD2 | 200 | 30 min | 10 ng/ml | 120–250 pg/ml |
| Cytokine | 1×105 | 6 h | 100 ng/ml (option: SCF 100 ng/ml) | IL-8: 200–600 pg/ml GM-CSF: 50–100 pg/ml |
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