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. Author manuscript; available in PMC: 2010 Nov 16.
Published in final edited form as: J Neurobiol. 2006 Oct 1;66(12):1365–1377. doi: 10.1002/neu.20315

Figure 3. Synapse elimination is unaltered in TIMP-2−/− mice.

Figure 3

A) Synapse elimination was determined by quantitating the percent of monoinnervated endplates in the diaphragm. Monoinnervated endplates in wild-type controls and TIMP-2−/− littermates is similar at P3 (45.6 ± 0.5% vs. 43.3 ± 2.0%; p = 0.3, n = 3), P7 (74.4 ± 1.9% vs. 79.1 ± 4.2%; p = 0.3, n = 4), and P14 (93.7 ± 1.9% vs. 94.0 ± 1.3%; p = 0.9, n = 3). B) Confocal photomicrographs of P3 diaphragm demonstrate that the number of axons per polyinnervated endplate is also comparable in wild-type and TIMP-2−/− mice. C) Multiphoton micrograph demonstrating the nerve terminal with FM1-43 (green) and the AChR endplate region with rhodamine-conjugated α-BTX (red) indicates that pre- and post-synaptic NMJ components are appropriately aligned in the diaphragm of P21 wild-type and TIMP-2−/− mice. Scale bars = 50 μm (B), 25 μm (C).