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. Author manuscript; available in PMC: 2010 Nov 16.
Published in final edited form as: J Neurobiol. 2006 Oct 1;66(12):1365–1377. doi: 10.1002/neu.20315

Figure 6. β1 integrin expression is reduced in TIMP-2−/− fast-twitch muscle.

Figure 6

A) Immunohistochemistry was performed on perfusion fixed, longitudinal cryosections. TIMP-2 co-localizes with β1 integrin at costameres in P21 EDL (A-C) and to a lesser extent adult EDL (G-I). In the soleus, TIMP-2 and β1 integrin are both expressed in the basal lamina. In addition, TIMP-2 is abundantly expressed in capillary endothelial cells at P21 (D-F) and in the adult (J-L). Scale bar = 25 μm. B) Western blot analysis with 25 μg protein from wild-type and TIMP-2−/− EDL. Densitometry was performed and the relative expression of β1 integrin was normalized to α-tubulin (n=3). The developmental decline in β1 integrin expression that normally occurs in wild-type muscle is much more precipitous in TIMP-2−/− muscle. C) Immunohistochemistry confirms the western blot analysis. β1 integrin expression at P21 is significantly decreased in the EDL and only moderately decreased in the soleus (n=3). Scale bar = 50 μm.