Figure 2.

Relative expression (Ct) ratios between polyploid and diploid organ samples of individuals of the northern distribution of the complex, for the ef1a, gapdh, rpl8 and β-actin genes. Expected ratio of 1.5 in the case of no silencing 3n/2n (dashed line), expected ratio of 2, in the case of no silencing 4n/2n (dotted line) and expected ratio of 1 in the case of silencing (solid line). Observed 3n to 2n ratio (dark grey columns), observed 4n to 2n ratio (light grey columns), number of triploid and tetraploid triplicates analysed for each ratio calculation (N).