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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Int J Mol Med. 2010 Dec;26(6):935–940. doi: 10.3892/ijmm_00000545

Figure 5.

Figure 5

Effect of chamomile on NF-κ B activity. (A) Western blot analysis for protein expression of p- IKKα /β , Iκ Bα and its phosphorylation, and RelA/p65 in the cytosolic fraction of RAW 264.7 macrophages stimulated with 1μg/mL LPS and LPS and chamomile for indicated doses, (B) RelA/p65 expression in the nuclear fraction, (C) EMSA assay. EMSA was performed to determine the effect of chamomile on the nuclear translocation of NF-κ B dimers and their binding to DNA. Controls: #1 Biotin-EBNA control DNA, #2 Biotin-EBNA control DNA+EBNA extract, #3 Biotin-EBNA control DNA + EBNA extract + 20-fold molar excess of unlabeled EBNA DNA. L-NMMA, a non-selective inhibitor of all NOS isoforms was used as positive control. The details are described in ‘Materials and Methods’ section.