Figure 7.

Cytosolic distribution of ATP7A and ATP7B in COS-1 cells following infection with viral vector in the presence of basal or added CuCl₂. COS-1 cells were infected with optimal rAdATP7Amyc or rAdATP7Bmyc viral titers as explained in Experimental Procedures. ATP7A (A and C) and ATP7B (B and D) were immunostained with the primary anti-myc tag monoclonal antibody and anti-mouse Alexa 488 secondary antibody (colored green). The nuclei are stained with propidium iodide (colored red). It is shown that, in the absence of added copper, the expressed ATP7A (A) and ATP7B (B) proteins (green) are mostly visible near the nuclei (red color), corresponding to the Golgi and trans-Golgi network. In the presence of added copper, ATP7A (C) is partly retained near the nuclei and partly targeted to plasma membranes; no trafficking vesicles are observed. On the other hand, ATP7B (D) is associated with numerous trafficking vesicles throughout the cytoplasm, with no significant plasma membrane association. The bar is 10 μm.