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. Author manuscript; available in PMC: 2010 Nov 16.
Published in final edited form as: Thromb Haemost. 2010 Jul 20;104(3):531–535. doi: 10.1160/TH09-12-0860

Fig. 2.

Fig. 2

Expression and functional characterization of the human ADAMTS13 wild type (WT) and intron retention isoforms (IR-25). (A) Western blot analysis of WT and IR-25 protein expression 24 hours following transient transfection of HEK293 cells. Thirty μg total protein of whole cell lysates or concentrated media were loaded on the gel. β-Actin levels in lysate samples are shown to be comparable (lower panel). (B) Fluorescogenic activity assay of intracellular WT and IR-25 over the course of a 60-minute incubation.