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SIVmac251-derived env clones represent a diverse array of sequences. The unrooted neighbor-joining tree shows nucleotide diversity among the 20 cloned SIVmac251 envelope sequences characterized in this study (branches are labeled a through t). The red cluster depicts SIVmac251 sequences from an expanded SIVmac251 challenge stock characterized by Keele et al.18 The env sequence of SIVmac239 was included as a reference.
