Comparative neutralization of SIV239, SIV316, and SIV239/Env251 clones by a pool of SIV-positive sera. Virus was incubated with serial 4-fold dilutions of sera and then C8166-45 SIV-SEAP cells were added. SEAP activity was measured 4 days postinfection. The viruses were assayed in triplicate. The relative standard deviation for the 17 recombinant cloned viruses was calculated, and ranged from 1% to 71%, with an average relative standard deviation of 12%. Clones SIV239/251c, SIV239/251r, and SIV239/251s were not infectious in SEAP cells and were not included in this assay.