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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Aug 11;30(10):2005–2013. doi: 10.1161/ATVBAHA.110.209908

Figure 4. RvE1 counter regulates P2Y12 signaling.

Figure 4

ADP (10−10 M – 10−4 M) added to CHO-P2Y12 (1×105 cells/well), RvE1 (10−10 M – 10−6 M), LTE4 (10−12 M – 10−8 M) or LTD4 (10−12 M – 10−8 M) were added (60 minutes at 37°C). All incubations were stopped with lysis buffer containing emerald substrate reagent for (60 minutes, 21°C). EC50 values were determined by curve fit analysis using GraphPad software (see methods). (A) ADP (squares) and RvE1 (triangles) dose response. Values are mean ± SEM of n=5. (B) RvE1 (10−10 M – 10−7 M), when added simultaneously with ADP. (C) LTE4 (circles) and LTD4 (triangles) dose response. Values are mean ± SEM of n=6. (D) LTE4 (10−9 M – 10−8 M) followed by ADP (10−5 M). Values are mean ± SEM , n=3, *p<0.05.