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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Aug 11;30(10):2005–2013. doi: 10.1161/ATVBAHA.110.209908

Figure 5. RvE1 regulates P2Y12 signaling in a ChemR23-dependant manner.

Figure 5

(A,B) CHO-hP2Y12β-arrestin cells were transiently transected with mock-pcDNA3 or hChemR23 using FuGene transfection reagent for 48 hours, 37°C. Cells were plated (1×105 cells/well), and ADP (10−5 M) or ADP plus RvE1 (10−10 M – 10−8 M) were incubated for 60 minutes, 37°C. (A) Human ChemR23 transient transfection was monitored using flow cytometry. Histograms are representative of n=4. (B) Inhibition of P2Y12 by RvE1 was monitored in mock-pcDNA3 (grey bars) or hChemR23 (black bars) Values are mean ± SEM of n=4, * p<0.03, 2-way ANOVA with Bonferroni post testing. (C) Proposed Scheme for RvE1 actions on human platelets (see discussion for details).