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. 2010 Sep 24;29(21):3723–3732. doi: 10.1038/emboj.2010.238

Figure 2.

Figure 2

Mapping the domains that mediate recruitment of TopBP1 to IR-induced 53BP1 or RPA foci. (A) Diagram of human TopBP1 showing the BRCT domains, numbered 1–8, and the ATR activation domain (AD) and summary of the properties of the tested TopBP1 deletion mutants. The mutants were scored according to efficiency of focus formation: +, wild type; /, foci formed, but a significant amount of TopBP1 remained in the nucleoplasm; −, no foci. (B, C) Examples of the localization of selected GFP-tagged TopBP1 mutants in U2OS cells 4 h after exposure to IR (9 Gy). The numbers refer to the residues present in the TopBP1 mutants. Δ, deletion.