Figure 3.
MPP8 is important for tumour cell proliferation. (A) Western blot analysis of two immortalized human mammary epithelial cells (MCF10A and hMEC-hTERT-LT) and six breast cancer cell lines (top panels); human normal fibroblast cells (WI-38) and six non-small cell lung cancer cells (bottom panels). (B) MPP8 knockdown results in decreased cell proliferation. Top panels are western blot analysis of control and two stable MPP8 knockdown MDA-MB-231 cell lines. Bottom panel shows the growth curve of different MPP8 knockdown cells. Viable cells were quantified every 24 h for 4 days after initial seeding. Cell numbers from six independent experiments were averaged, and the variations are presented with error bars.