Table 1.

Actions of NF-κB inhibitors on deoxycholyltaurine (DCT)-induced NF-κB nuclear translocation and transcriptional activation in H508 colon cancer cells

Inhibition, % reduction observed with inhibitor plus DCT (100 μM)
Inhibitor	Nuclear translocation	p	Transcriptional activation	p
SN50 (20 μg/ml)	92.4±10.2	0.01	75.8±6.8	0.02
MG-132 (50 μM)	85.4±8.7	0.01	82.7±7.3	0.02
BAY11-7082 (10 μM)	70.9±6.4	0.02	60.4±6.1	0.03

H508 cells were incubated for 30 min with 100 μM DCT, alone or with the indicated concentration of NF-κB inhibitor, and nuclear extracts were immunoblotted for the p65 NF-κB subunit. NF-κB transcriptional activation was measured by using a TransAM NF-κB kit. Values are means±SE from three experiments. p indicates comparison of values observed with cells incubated with DCT (100 μM) plus the indicated concentration of NF-κB inhibitor compared to cells incubated with DCT alone.