Table 1. Reactivity of IgM and IgG antibodies directed against JEV in ELISA and IIFT.
anti-JEV IgM | anti-JEV IgG | ||||
Sera positive by Panbio ELISA/sera tested [Panbio Units range] | Sera positive by InBios ELISA/sera tested [ISR range] | Sera positive by Euroimmun IIFT/sera tested [titre range] | Sera positive by InBios ELISA/sera tested [ISR range] | Sera positive by Euroimmun IIFT/sera tested [titre range] | |
PRNT50 positive vaccinees | 50/78 (64%) [0.9–55] | 56/78 (72%) [1.4–59.9] | 51/78 (65%) [0–320] | 6/91 (7%) [0.7–12.4] | 91/97 (94%) [0–1000] |
PRNT50 negative vaccinees | nd | nd | 0/78 (0%) | nd | 0/78 (0%) |
anti-DENV positive patients | 1/10 (10%)+ [2.0–21.3] | 5/10 (50%) [3.5–17.8] | 3/15 (20%)* [0–32] | 17/20 (85%) [1.4–27.1] | 20/20 (100%)* [10–1000] |
German blood donors | nd | nd | 4/200 (2%) [0–10] | nd | 8/197 (4%) [0–32] |
nd: not done.
ISR: InBios immune status ratio;
+: calculation of JE/dengue ratio can be used to determine the presumptive infection;
*incubation on Flavivirus Profile 2 can be used to determine the presumptive infection.