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In the 1,2-propanediol utilisation metabolosome the adenosylcobalamin-dependent diol dehydratase (PduCDE) occasionally generates aquacobalamin. This inactivated form of the coenzyme is reactivated by the action of a corrin reductase, PduS, and an adenosyltransferase (PduO). This is achieved by two sequential one-electron reductions of aquacob(III)alamin to cob(I)alamin prior to the transfer of the adenosyl moiety from ATP.
