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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Cancer Res. 2010 Sep 23;70(22):9402–9412. doi: 10.1158/0008-5472.CAN-10-1199

Figure 4.

Figure 4

Elevated ANCCA is required for proliferation and survival of triple-negative breast cancer (TNBC) cells. A and B, Cells were either infected with equal titers of adeno-ANCCA or adeno-control (HCC1937) or transfected with siRNA targeting ANCCA or control sequence (MDA-MB 468). Western and cell proliferation were performed with cells harvested 48 hrs later (for Western) or at indicated days. C, MDA-MB 231 cells were transfected with siRNA-ANCCA or siRNA-control and plated in soft agar for colony formation or Western. Colonies were stained with MTT four weeks later and counted. Representative images of colonies and their numbers from different treatments are shown. D, MDA-MB 231 cells were processed for TUNEL assay (left panel) or Western, at day 3 after siRNA transfection. Paired samples t-test (for A and B) or independent samples t-test (for C and D) was used.