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. Author manuscript; available in PMC: 2011 Dec 9.
Published in final edited form as: Mol Microbiol. 2010 Jun 9;77(4):912–929. doi: 10.1111/j.1365-2958.2010.07255.x

Figure 7. Invasion and gliding assays of mic6ko and complemented strains. The penultimate tryptophan residue in the CTD of TgMIC6 is not critical for productive invasion.

Figure 7

A. IFA documented by confocal microscopy of intracellular parasites deficient in TgMIC6 (mic6ko) and complemented with TgMIC6-Ty (mic6ko/MIC6Ty) or MIC6W/ATy (mic6ko/MIC6W/ATy). Co-localization with the micronemal marker TgMIC4 was assessed using antibodies anti-TgMIC4 (red) and anti-Ty-1 (green). Scale bars indicate 5μm.

B. Quantification of the relative invasion efficiency of the four parasite strains as determined by a cell invasion assay normalized to RH-YFP strain co-cultivated and used as internal standard for parasite fitness. Error bars indicate standard deviations.

C. Gliding assays of mic6ko and mic6ko/MIC6W/ATy parasites. The trails were stained with anti-SAG1. The arrow indicates a trail.