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. Author manuscript; available in PMC: 2011 Nov 23.
Published in final edited form as: Biochemistry. 2010 Oct 29;49(46):9972–9984. doi: 10.1021/bi1014858

Figure 8. Side chain structure and hydrophobicity of amino acids at 594, 595 and 617 are important for the constitutive activation of Jak2-V617F.

Figure 8

(A) Autophosphorylation of the indicated Jak2 plasmids was assessed by western blot analysis as described previously (top). The same membrane was stripped and re-probed for total Jak2 (bottom). (B) Jak2 autophosphorylation was quantified using densitometry and the average values from at least three independent experiments were plotted as a function of Jak2 mutation status, * p < 0.05. (C) Luciferase assays were conducted using COS-7 cells transfected with the indicated Jak2 expression plasmids. The average luciferase activity (RLU) for each construct from at least three independent experiments was plotted as a function of Jak2 mutation status, * p < 0.05.