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| Recovery of ACAT enzyme activity during purification | |
|---|---|
| Whole cell extract | 100% |
| Solubilized enzyme | 97 |
| Nickel column chromatography | 21 |
| Flag column chromatography | 32 |
| Total recovery | 7 |
| Total ACAT unit (nmole/min) | 118 |
| Total ACAT1 protein recovered | 250 μg |
55 of 145 cm2 dishes of H293 cells stably expressing HisACAT1/Flag were employed as the starting material.
