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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Dev Biol. 2010 Oct 20;348(2):177–189. doi: 10.1016/j.ydbio.2010.09.019

Fig. 10. Lipid profiles of FABP9−/− and WT sperm.

Fig. 10

(A) Total lipids were extracted from sperm and developed on a silica gel TLC plate using 60:25:4 (chloroform:methanol:water) as the solvent system. Lipid standards indicated are as follows. (1) Polar lipid mix: cholesterol, phosphatidyl ethanolamine, lecithin, lyso-lecithin; (2) Sphingolipid mix: cerebrosides, sulfatides, sphingomyelin; (3) Fatty acid mix: methyl palmitate, methyl stearate, methyl oleate-9, methyl linoleate-9,12, methyl linoleate-9,12,15; (4) Poly-unsaturated fatty acid (PUFA) mix: C14, C16, C16:1n-7, C18:1n-7, C18:1n-9, C18:2n-6, C20:1n-9, C18:4n-3, C22:1n-11, C20:5n-3, C22:1n-9, C22:5n-3, C22:6n-3. Fluorescent bands were imaged after spraying with a primuline solution. (B) Quantitative profiles of sperm fatty acids were determined by covalent adduct chemical ionization tandem mass spectrometry and calculated using methyl-17:0 as an internal standard. Detectable fatty acids were 14:0, 16:0, 18:0, 18:1n-9, 18:2n-6, 20:3n-6, 20:4n-6, 22:5n-3 and 22:6n-3. There were no significant differences in fatty acid levels between WT and FABP9−/− sperm.