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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Dev Biol. 2010 Oct 20;348(2):177–189. doi: 10.1016/j.ydbio.2010.09.019

Fig. 9. Membrane organization and signaling in FABP9−/− mice.

Fig. 9

Localization in FABP9−/− sperm of (A) glycosphingolipid GM1 and (B) focal enrichments of membrane sterols. EGFP-conjugated D4 of perfringolysin O (PFO-D4) was used to localize focal enrichments of sterols and CTB was used to localize GM1. These segregations to the plasma membrane overlying the acrosome were consistent both with previous reports (Selvaraj et al., 2006; Selvaraj et al., 2009) and with WT cohorts (not shown). (C) Tyrosine phosphorylation of FABP9−/− and WT sperm proteins after exposure to stimuli to induce capacitation. Capacitation-associated phosphorylation profile of sperm proteins was not affected in FABP9−/− sperm compared to WT. [NC: non-capacitated; C: capacitated]