Table 1.
PCR primers sequences used in this study.
| Gene | Transcript ID | Primer | Sequence | Product |
|---|---|---|---|---|
| UCN 1 | ENSCAFT0000003593 7 |
Sense Antisens e |
5′-AGGACCCAAGTCTGCGCTA 5′- CGAATATGATGCGGTTCTGCTC |
258 bp |
| UCN 2 | ENSCAFT0000001979 0 |
Sense Antisens e |
5′-ATGACCAGGTGGGCTTTG 5′-GTTGGTGGTGGCCTGCTC |
306 bp |
| UCN 3 | ENSCAFT0000000847 3 |
Sense Antisens e |
5′- CAGAAGTTCCACCCAGGAAA 5′-GTATTTGTACCGGGCGCTTA |
240 bp |
| CRFR2α | ENSCAFT0000000497 1 |
Sense Antisens e |
5′-AGGAGCTGCTCCTGGACGG 5′-TGCGGTAGTGCAGGTCATAC |
285 bp |
| CRFR2β | ENSCAFT0000000497 2 |
Sense Antisens e |
5′-GAGCCAGGCGCAGATACAT 5′- AAACGCAGTGACCCAGGTAG |
356 bp |
| S16 | ENSCAFT0000000550 1 |
Sense Antisens e |
5′-CAAGGGTCCTCTGCAGTCC 5′-GTCCCATGACAGCGGTTTAT |
485 bp |