Skip to main content
. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Cancer Res. 2010 Sep 30;70(22):9137–9142. doi: 10.1158/0008-5472.CAN-10-1684

Figure 1.

Figure 1

RNA co-immunoprecipitation using anti-Argonaute (AGO) with downstream microarray analyses with Affymetrix Human Gene ST 1.0 (RIP-Chip) shows that multiple members of the miR-15/107 miRNA group recruit GRN mRNA to the AGO-microribonucleoparticle (miRNP). The vast majority of mRNAs (β-Actin is representative) were not enriched in the miRNP following miR-107 transfection. The RIP-Chip results from different transfections can be compared (each performed in triplicate; miRNA sequences shown at bottom). The negative control miRNA is indicated on the right side with the black arrow. Only after transfection with miR-103, miR-107, miR-16, miR-195, and MUT2, which all have common 5' sequence AGCAGCA motif, GRN mRNA is recruited to the AGO-miRNP. Statistical tests were performed using the Student's t-test with a P<0.01 cutoff (green asterisk indicates significant difference versus the control miRNA transfection).