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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: Int J Cancer. 2011 May 15;128(10):2481–2494. doi: 10.1002/ijc.25587

Figure 3. DMAPT upregulates p73 and p21 and alters cell cycle distribution in a cell dependent manner.

Figure 3

(A) RT-PCR demonstrated DMAPT mediated upregulation of p73 in UMUC-3 (left panel) and small interference (si) RNA inhibition of p73 transcription was demonstrated by Western blot of UMUC-3 cell line with a non-target control and with and without DMAPT (right panel). (B) Western blotting demonstrated DMAPT upregulated p21 in UMUC-3 with non-target siRNA, and showed that this was partially blunted in the siRNA-p73 cell line variant (left panel). Western blotting also showed that NAC partially blocked DMAPT mediated upregulation of p21(right panel). (C) RT-PCR detailed DMAPT's ability to increase p73 (left panel) and p21 in NSCLC cell line, A549 (right panel). (D) Flow cytometric analysis demonstrated DMAPT induced accumulation of cells in S phase at 12 and 24 hours in UMUC-3 (top panel) and G2 phase at 12 hours but not 24 hours in A549 cells (bottom panel) . NAC blocked cell cycle effects of DMAPT in both cell lines.