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. 2010 Nov 15;191(4):771–781. doi: 10.1083/jcb.201005140

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© 2010 Ludwig et al.

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Figure 3. — Flotillin microdomains associate with myosin IIa and spectrins. (a) Protocol for isolation of flotillin microdomains. (b) Identification of proteins associated with flotillin microdomains in HeLa cells by mass spectrometry. The specific bands from Coomassie-stained gels indicated with arrows were identified by mass spectrometry. Numbers in brackets indicate the number of unique peptides identified. MW, molecular weight (kD). (c) Confirmation that α-spectrin and myosin IIa associate specifically with flotillin microdomains in HeLa cells. Immunoisolation was performed as in panels a and b above; samples were analyzed by Western blotting with antibodies against the proteins indicated.