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. 2010 Nov 15;191(4):795–808. doi: 10.1083/jcb.201006028

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© 2010 Woodruff et al.

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Figure 7. — Kip3 and Ipl1 operate in separate pathways during spindle disassembly. (A) Frequency in which spindles disassembled after initiation of cytokinetic ring contraction in wild-type (WT) cells and selected mutants (n = 3 separate experiments each analyzing 20 disassembly events for each strain). All cells were incubated at 37°C. (B) Analysis of Kip3-13Myc phosphorylation in wild-type and ipl1-321 strains. Protein extracts were separated by SDS-PAGE supplemented with 20 µM Phos-tag acrylamide then visualized by immunoblotting against the Myc epitope. (C) Quantification of delayed spindle disassembly in wild-type cells (n = 60) and in cells expressing an allele of KIP3 with all four Ipl1 consensus sites mutated (kip3-4A; n = 26). (D) Kip3 regulates mitotic spindle length throughout anaphase. Spindle length was measured in kip3Δ or wild-type cells expressing GFP-Tub1 arrested in early anaphase (cdc14-2) or late anaphase (cdc15-2; n = 60 for each strain). Error bars indicate mean ± SD.