Table I.

Depolymerization kinetics of spindle halves after onset of spindle disassembly

Genotype	Recovery frequency	Shrinkage rate	n	P-value
	F(rec) (s−1)	R(hs) (µm × s−1)
23°C
Wild type	0.0050	0.069 ± 0.006	42	NA
cdh1Δ nonhyperstable	0.0054	0.051 ± 0.007	21	0.085
cdh1Δ hyperstable	0.0046	0.013 ± 0.004	16	<0.001
doc1Δ nonhyperstable	0.0064	0.044 ± 0.006	25	0.013
doc1Δ hyperstable	0.0016	0.004 ± 0.002	16	<0.001
dbf2Δ nonhyperstable	0.0079	0.029 ± 0.004	19	<0.001
dbf2Δ hyperstable	0.002	0.012 ± 0.002	16	<0.001
kip3Δ	0.0102	0.044 ± 0.004	39	0.002
dcc1Δ	0.0078	0.043 ± 0.004	40	0.003
ctf8Δ	0.0082	0.041 ± 0.005	39	0.008
she1Δ	0.0053	0.048 ± 0.004	45	0.008
mcm21Δ	0.0059	0.060 ± 0.005	42	0.31
37°C
Wild type	0.0028	0.090 ± 0.007	40	NA
ipl1-321	0.0045	0.052 ± 0.004	36	<0.001
kip3Δ	0.0125	0.061 ± 0.009	19	0.014
ipl1-321 kip3Δ	0.0076	0.042 ± 0.010	17	<0.001

NA, not applicable. Cells with indicated the genotypes expressing GFP-Tub1 were imaged every 10 s, and the lengths of the spindle halves were measured at each time point. A recovery event was scored each time a spindle-half switched from shrinkage to growth. The shrinkage rate (R(hs)) represents the slope of the half-spindle lengths when plotted versus time (mean ± SEM). P-values were calculated using a model I analysis of variance test. Half-spindle shrinkage rates for each mutant were directly compared with the wild-type rates.