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. 2010 Nov 15;191(4):721–729. doi: 10.1083/jcb.201006049

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© 2010 Hatch et al.

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Figure 2. — Cep152 localizes to centrioles throughout the cell cycle. (A) RPE-1 cells at the indicated cell cycle stages were fixed and stained for Cep152, centrin, or polyglutamylated (poly-glu) tubulin, which marks centrioles and primary cilia, and DNA. Insets show enlarged centrosomes. Bars, 5 µm. (B) G1 centrosomes in a U2OS cell stained for Cep152, centrin, and γ-tubulin. (C, top) Centrosomes in a U2OS cell stained for Cep152, Sas6, and γ-tubulin. (bottom) Centrosome in a RPE-1 cell overexpressing Plk4 stained for centrin, Sas6, and Cep152. (B and C) Images are maximum projections of deconvoluted image stacks. Bars, 1 µm.