Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Nov 15;191(4):731–739. doi: 10.1083/jcb.201007107

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Cizmecioglu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — CPAP localization at the centrosome is dependent on Cep152. (A) U2OS cells were transfected with either GL2 or Cep152 siRNAs. Colocalizations of CPAP, hSas6, Cep135, and CP110 (red) together with γ-tubulin (green) were determined by immunofluorescence. Insets show enlargements of the merged image and individual channels. (right) Protein levels of the indicated proteins were determined by Western blotting. The graph shows a quantification of the percentage of cells with centrosomal CPAP localization. Error bars indicate SDs (n = 3). (B, left) Cep152 and CPAP costainings (red) within the flower-like centrin-2 structures (green) were depicted. (B, right) Cep152 and CPAP colocalizations (red) were performed together with HA-Plk4 (green) using anti-HA antibodies. (C) Flag-CPAP and GFP or GFP-Cep152 constructs were coexpressed in 293T cells. GFP and GFP-Cep152 were immunoprecipitated 48 h after expression. Coimmunoprecipitated Flag-CPAP was detected by Western blotting against the Flag tag. (D) Different GFP-Cep152 fragments (Fig. S3 A) were coexpressed with Flag-CPAP in 293T cells. Anti-GFP immunoprecipitates were analyzed by Western blotting for coprecipitated Flag-CPAP with anti-Flag antibodies. Bars: (A) 5 µm; (B, left) 1 µm; (B, right) 0.5 µm.