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. Author manuscript; available in PMC: 2010 Nov 17.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 18;374(3):485–489. doi: 10.1016/j.bbrc.2008.07.043

Figure 2.

Figure 2

SDS-PAGE gels (12%) of fractions obtained during the purification of YdcS from periplasm of E. coli BW25113 cells . The gels were stained with Coomassie Blue. Lane 1: 45% ammonium sulfate precipitant; lane 2: active fractions from HiPrep 16/10 phenyl FF column chromatography; lane 3: active fractions from HiPrep 16/10 Q FF column chromatography; lane 4: YdcS after Superdex 75 column chromatography; lane 5: YdcS-GFP purified by the same sequence after Superdex 75 chromatography.