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cOHB concentration of whole cells and cell fractions of E. coli BW25113 (wild-type) and JWK1435-1 (ydcS deletion mutant 1) determined by chemical assay. Cytoplasm includes periplasm. Values are the average of 2 determinations.
| E. coli cells | Concentration of cOHB | |||
|---|---|---|---|---|
| Whole Cells (µg/mg dry wt.) |
Cytoplasm (µg/mg prot) |
Inner Membrane (µg/mg prot) |
Outer Membrane (µg/mg prot) |
|
| BW25113 (WT) | 5.0 | 1.5 ± 0.2 | 1.4 ± 0.2 | 3.0 ± 0.4 |
| JWK1435-1 (DM1) | 3.7 | 1.4 ± 0.2 | 1.3 ± 0.2 | 2.1 ± 0.3 |
