Table 1.

Synthesized caged and non-caged restriction enzyme templates.^[a]

DNA	Sequence	Tm/°C
T0	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	68.0 ± 0.7
T1	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	62.8 ± 1.1
T2	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	62.5 ± 0.8
T3	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	62.5 ± 0.7
T4	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	63.5 ± 0.4
T5	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	63.3 ± 0.3
T2,3,6	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	50.8 ± 1.2
T4,5	5’ GGGTGAATTCAGATCTGGATCCAAAAG 3’	59.5 ± 0.7

^[a]Melting temperature of PS DNA/DNA hybrids. T denotes the caged thymidine.