Figure 2. The changes in deuterium incorporation in peptic peptides in a) ATP-bound vs. apo-Arp2/3 complex and b) ATP- vs. ATP+WASp-Arp2/3 complex.
Peptides from each subunit are plotted against the difference in deuterium incorporation following an 80 minute HDX. Differences in deuterium incorporation were determined by the mass difference in individual peptides between a, the apo- and the ATP-bound complex and b, the ATP- and ATP/WASp-bound where negative numbers indicate enhanced protection when ligand is bound, while positive numbers indicate decreased protection. a, Arp3; b, Arp2; c, ARPC1; d, ARPC2; e, ARPC3; f, ARPC4; g, ARPC5.