Figure 1.

The protein sequence of type II human hexokinase (P52789) was submitted to emotif-search online. The cutoff expectation for the query was set at 1e^–7. The matches were ranked in order, so that matches with the more specific emotifs were reported first. Along with each hit, emotif-search calculates the expectation for the query based on the fraction of the emotif database it has already run against the input sequence. In addition emotif-search determines the probability that the query sequence has not matched a single emotif from the ones processed so far by chance. The reporting of these two values allows the user to rerun the search with a lower value of the cutoff expectation for a given query. The black line between the sixth and the seventh emotif match is a sample readjustment performed on this particular sample query. The cutoff expectation was lowered from 1e^–7 to 1e^–15. Only the first six hits were considered significant by the new, more stringent cutoff.