Fig. 3.

MATS relative potency (RP) of NHBA, NadA, and fHbp determined by the MATS ELISA in antigen-specific subpanels (strains mismatched for the other three antigens, A, B, and C, respectively) and in the 57-strains panel (D, E, and F, respectively). The strains are in ascending order of MATS RP. Strains covered (as determined by killing in the SBA with pooled postimmune infant sera) are shown with solid red circles, and those not covered are shown with solid blue circles. The dashed line represents the positive bactericidal threshold (PBT) for each antigen; this threshold is the relative potency above which at least 80% of MenB strains are killed in SBA with serum from vaccinated subjects. Error bars represent 95% confidence limits for each measurement, deduced from the intermediate precision of the method.