Fig. 5.

Properdin reconstitution in fB^−/− but not C3^−/− mice after injection of classical pathway-activating immune complexes (IC). (A) Effect of IC on properdin in fB^−/− mice. Peroxidase-rabbit anti-peroxidase IC (200 μg) were administrated i.v. to fB^−/− mice. Serum was harvested at 10, 30, and 60 min and at 3 and 24 h after injection. Each time-point represents a single mouse. (B) Effect of IC on properdin in C3^−/− mice. Activation of complement by IC in C3^−/− mice did not generate properdin in blood. Representative of three experiments each in A and B.