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. 2010 Oct 25;107(45):19332–19337. doi: 10.1073/pnas.1008248107

Fig. 4.

Fig. 4.

3′PIs regulate commitment to phagocytosis with different effects on early and late signals. (A) Effect of Bpv(pic) on the phagocytic index of B0.3 (n = 6; *P < 0.0001). (B) Effects of inhibitors on the phagocytic indexes of B1. Bpv(pic) increased phagocytic index, whereas LY294002 and TGX-221 decreased phagocytic indexes (n = 6; *P < 0.01, **P < 0.001, ***P < 0.0001). (C) Effect of LY294002 on the phagocytic index of B10 (n = 6; *P < 0.0001). Percentages indicate actual phagocytic indexes. (D–F) Recruitment of YFP-actin (D), YFP-AktPH (E), and PKCε-YFP (F) to cups in LY294002-treated cells (LY cups) and stalled cups and early (early cup) and maximal values (max) for successfully formed B1 phagosomes. Early cups were similar in size and shape to stalled cups, using frames from early time points of successful phagocytic events. YFP-AktPH recruitment to early cups was greater than recruitment to stalled cups (n = 5; *P = 0.045). LY294002 did not inhibit the recruitment of YFP-actin (D; n = 3) but inhibited the recruitment of YFP-AktPH (E) and PKCε-YFP (F; n = 5).